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KMID : 1123920030170051251
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 5 p.1251 ~ p.1256
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Effect of Zibachunggan-tang on lipopolysaccharide-induced expression of NF-kB downstream genes in HepG2 cell
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Hong Sang-Hoon
Choi Byung-Tae
Lee Yong-Tae
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Abstract
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To determine the effect of Zibachunggan-tang(ZCT) on the process of lipopolysaccharide (LPS)-induced nuclear factor-kBp65 (NF-kBp65) activation, and LPS-induced expression of pro-inflammatory proteins including tumor necrosis factor-α (TNF-α), nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in HepG2 cell. Immunoblot analysis showed that the level of nucleic NF-kBp65 was rapidly up-regulated and cytosolic inhibitory I-kBα was down-regulated by LPS challenge. While ZCT inhibited an increase of NF-kBp65 and degradation of I-kBα in HepG2 cell. Beside LPS-induced expression of a group of genes, such as TNF-α, inducible iNOS and COX-2, are repressed by ZCT. It may be concluded that ZCT attenuates the progress of LPS-induced inflammation by reduction of NF-kBp65 activation. The ZCT would be useful as a therapeutic agent for endotoxin-induced liver disease.
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KEYWORD
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Zibachunggan-tang(ZCT), LPS, NF-kBp65, cyclooxygenase-2 (COX-2), HepG2 cell
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